Schedule
FEBS PC2024 program
June 09-15, 2024
Faculty of Science University of South Bohemia Ceske Budejovice Czech Republic
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Lectures 8:30–12:30 7:45-8:30 Breakfast 13:00-14:00 Lunch |
Lab Exercises 14:00–19:00 15:30 Coffee and tea time 19:00 Dinner (Tue, Thu) |
Evening events 20:00 |
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Sunday June 09 |
18:00-20:00 Registration |
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Monday June 10 morning |
9:00-12:00 Registration 10:00-12:00 practical WS2 - Exploring the Invisible World with Nikon and Nanolive [Barbora Kobidová] |
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Monday June 10 afternoon |
12:00-12:15 Welcome and Course remarks [Ivana Kutá Smatanová & Pavlína Řezáčová] 12:15-12:30 Prologue by the USB representative – [Luděk Berec] Prologue by the FSci USB representative – dean of FSci [František Vácha] 12:30-12:45 Introduction of FEBS activities by the FEBS ACC representative [Mutay Aslan] 12:45-13:15 Tackling the reproducibility crisis in scientific research [Sara Fuentes, Managing Editor of FEBS Open Bio] (on-line) 13:15-13:35 Greeting speech by Rolf Hilgenfeld (on-line) Greeting speech by Juan Ma. Garcia-Ruiz (on-line) 13:35 Coffee and tea time
14:05-14:45 AlphaFold and biochemical considerations for protein crystallization [Joe Ng] 14:45-15:30 InCellCryst - A streamlined approach for protein crystallization in living cells [Lars Redecke] 15:30-16:15 Crystallization of membrane proteins in lipidic systems [Martin Caffrey] 16:45-17:30 Synthetic macrocycles as mediators of protein crystallization [Peter Crowley] From target structures to drugs [Andrea Brancale] The Chemistry of Mushroom Magic [Bernhard Rupp]
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Monday June 10 evening |
20:00 Welcome party |
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Tuesday June 11 morning |
8:30-9:15 From protein solution to crystals: Nature and formation of protein crystals [Bernhard Rupp] 9:15-10:00 From protein expression and purification to crystallization [Sergio Martínez Rodríguez] 10:00-10:30 Crystallization for the desperate [Terese Bergfors] 10:30 Coffee and tea time 10:45-11:25 Hofmeister ion series and the protein phase diagram [Jeroen Mesters] 11:25-12:05 Protein crystallization by capillary counter-diffusion methods [Jose A. Gavira] 12:05-12:45 Construct design and limited proteolysis strategies [Jerome Basquin] 12:45-13:00 A Crystallographer’s guide to the Galaxy [Paul Driver, Molecular Dimensions] |
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Tuesday June 11 afternoon |
1. Intracellular protein crystallization [L. Redecke] 2. Conventional techniques and crystallization of own proteins and [J. Mesters] 3. Crystallization of membrane proteins in lipidic system [M. Caffrey] 4. Observation of crystal growth / Seeding [T. Bergfors] 5. Capillary protein crystallization using counter-diffusion techniques [J. Gavira] |
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Tuesday June 11 evening |
20:00 Discussion with speakers and tutors of the day + posters section I Theory of X-ray diffraction I. [Jeroen Mesters] |
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Wednesday June 12 morning |
8:30-9:15 Latest Methods in scoring and optimizing sample suspensions prior to crystallization experiments and monitoring nucleation and crystal growth [Christian Betzel] 9:15-9:45 Protein as the main variable in crystallization [Ľubica Urbániková] 9:45-10:15 Unconventional crystallization strategies and techniques for screening and optimization [Lata Govada] 10:15-11:00 Microfluidics in action: crystallization and crystallography in microchips [Claude Sauter] 11:00 Coffee and tea time 11:15-11:45 What's this in my drop? Identifying drop phenomena". [Terese Bergfors] 11:45-12:30 Microseed Matrix Screening and its use in Structure Based Drug Discovery [May E. Sharpe] 12:30-13:00 Sample preparation for routine and advanced structural biology, including serial data collection, microED, and cryoEM [Patrick Shaw Stewart] |
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Wednesday June 12 afternoon |
1. Observation of crystal growth / Seeding [T. Bergfors] 2. Experimental phasing: practical considerations [J. Basquin] 3. "Random" Microseeding [P. Pachl, P. Shaw Stewart] 4. Capillary protein crystallization using counter-diffusion techniques [J. Gavira] 5. AlphaFold and biochemical considerations for protein crystallization [Joe Ng] 6. From the biomolecule solution to its 3D structure in a microfluidic chip [C. Sauter] Optional exercises: Crystallization under oil [L. Govada] |
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Wednesday June 12 evening |
Visit of town Ceske Budejovice and the evening in own direction |
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Thursday June 13 morning |
8:30-9:00 Using Fluorescence to Find Your Crystals [Crissy L. Tarver] 9:00-9:45 Crystallization Screening Results Analysis and Condition Prediction [Marc L. Pusey] 9:45-10:30 Crystallographic fragment-screening: workflow, tools and procedures [Manfred Weiss] 10:30 Coffee and tea time 10:45-11:30 Introduction to single particle analysis by cryo-EM [Oksana Degtjarik] 11:30-12:15 Sample preparation for single particle cryo-EM [Iuliia Iermak] 12:15-12:35 Advancements in Imaging Technologies and Microscopy: Exploring the Invisible World with Nikon and Nanolive [Barbora Kobidová, Zbyněk Halbhuber, Altium International] |
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Thursday June 13 afternoon |
1. Dynamic light scattering [K. Dierks, H. Brognaro] 2. Trace Fluorescent Labeling and Low Cost Fluorescent Imaging [M. Pusey, C. Tarver] 3. Crystal observation, testing, handling, mounting and cryocooling [ J. Brynda, P. Pachl] 4. Intracellular protein crystallization [L. Redecke] 5. Soaking and co-crystallization [B. Kaščáková] 6. Single particle cryo-EM [H. Luecke] 7. "Random" Microseeding [P. Pachl, P. Shaw Stewart] |
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Thursday June 13 evening |
20:00 Discussion with speakers and tutors of the day + posters section II Theory of X-ray diffraction II. [Jeroen Mesters] Poster prize awards |
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Friday June 14 morning |
8:30-9:15 Preparation of protein samples for crystallization experiments [Pavlína Řezáčová] 9:15-10:00 Preparation and crystallization of protein complexes: Tricks and examples from our host-virus studies [Ivana Nemčovičová] 10:00-10:45 Extremely brilliant X-ray sources and new opportunities in macromolecular crystallography [Petr Pachl] 10:45 Coffee and tea time 11:00-11:45 How to trap small objects in a beam of light [Dušan Novotný, MT-M]
12:00-12:10 12:10-12:20 Student presentation 2 12:20-12:30 Student presentation 3 |
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Friday June 14 afternoon |
15:30-16:15 16:30-19:00 Optional exercises: Crystal observation, testing, handling, mounting and cryocooling Conventional techniques and crystallization of own proteins [J. Mesters] Intracellular protein crystallization [L. Redecke] Trace Fluorescent Labeling and Low Cost Fluorescent Imaging [M. Pusey, C. Tarver] AlphaFold and biochemical considerations for protein crystallization [Joe Ng] |
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Friday June 14 evening |
19:30 Closing ceremony followed by Farewell dinner |
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Saturday June 15 morning |
Optional exercises: Crystal observation, testing, handling, mounting and cryocooling [P. Pachl, J. Brynda] Conventional techniques and crystallization of own proteins [J. Mesters] Intracellular protein crystallization [L. Redecke] Trace Fluorescent Labeling and Low Cost Fluorescent Imaging [M. Pusey, C. Tarver] AlphaFold and biochemical considerations for protein crystallization [Joe Ng] 12:00 Lunch take away and End of the course |
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