Schedule
FEBS PC2024 program
June 09-15, 2024
Faculty of Science University of South Bohemia Ceske Budejovice Czech Republic
|
Lectures 8:30–12:30 7:45-8:30 Breakfast 13:00-14:00 Lunch |
Lab Exercises 14:00–19:00 15:30 Coffee and tea time 19:00 Dinner (Tue, Thu) |
Evening events 20:00 |
|
Sunday June 09 |
18:00-20:00 Registration |
|
Monday June 10 morning |
9:00-10:00 and 12:00-13:00 Registration 10:00-12:00 practical WS [TBA] |
|
Monday June 10 afternoon |
13:00-13:15 Welcome and Course remarks [Ivana Kutá Smatanová & Pavlína Řezáčová] 13:15-13:30 Prologue by the USB representative – rector or vice-rector Prologue by the FSci USB representative – dean of FSci [František Vácha] 13:30-13:45 Introduction of FEBS activities by the FEBS ACC representative [Mutay Aslan] 13:45-14:15 Tackling the reproducibility crisis in scientific research [Sara Fuentes, Managing Editor of FEBS Open Bio] (on-line) Greeting speech by Rolf Hilgenfeld (on-line) Greeting speech by Juan Ma. Garcia-Ruiz (on-line) 14:35 Coffee and tea time 15:00-15:45 AlphaFold and biochemical considerations for protein crystallization [Joe Ng] 15:45-16:30 InCellCryst - A streamlined approach for protein crystallization in living cells [Lars Redecke] 16:30-17:15 Crystallization of membrane proteins in lipidic systems [Martin Caffrey] 17:15-18:00 Synthetic macrocycles as mediators of protein crystallization [Peter Crowley] 18:00-18:45 Medical chemistry [Andrea Brancale] |
|
Monday June 10 evening |
20:00 Welcome party |
|
Tuesday June 11 morning |
8:30-9:15 Principles of protein crystallization [Bernhard Rupp] 9:15-10:00 From protein expression and purification to crystallization [Sergio Martínez Rodríguez] 10:00-10:30 Crystallization for the desperate [Terese Bergfors] 10:30 Coffee and tea time 10:45-11:25 Hofmeister ion series and the protein phase diagram [Jeroen Mesters] 11:25-12:05 Protein crystallization by capillary counter-diffusion methods [Jose A. Gavira] 12:05-12:45 Construct design and limited proteolysis strategies [Jerome Basquin] 12:45-13:00 A Crystallographer’s guide to the Galaxy [Paul Driver, Molecular Dimensions] |
|
Tuesday June 11 afternoon |
1. Intracellular protein crystallization [L. Redecke] 2. Conventional techniques and crystallization of own proteins and [J. Mesters] 3. Crystallization of membrane proteins in lipidic system [M. Caffrey] 4. Observation of crystal growth / Seeding [T. Bergfors] 5. Capillary protein crystallization using counter-diffusion techniques [J. Gavira] 6. Crystallization under oil [L. Govada] |
|
Tuesday June 11 evening |
20:00 Discussion with speakers and tutors of the day + posters section I Theory of X-ray diffraction I. [Jeroen Mesters] |
|
Wednesday June 12 morning |
8:30-9:15 Latest Methods in scoring and optimizing sample suspensions prior to crystallization experiments and monitoring nucleation and crystal growth [Christian Betzel] 9:15-9:45 Protein as the main variable in crystallization [Ľubica Urbániková] 9:45-10:15 Unconventional crystallization strategies and techniques for screening and optimization [Lata Govada] 10:15-11:00 Microfluidics in action: crystallization and crystallography in microchips [Claude Sauter] 11:00 Coffee and tea time 11:15-11:45 What's this in my drop? Identifying drop phenomena". [Terese Bergfors] 11:45-12:30 Microseed Matrix Screening and its use in Structure Based Drug Discovery [May E. Sharpe] 12:30-13:00 Sample preparation for routine and advanced structural biology, including serial data collection, microED, and cryoEM [Patrick Shaw Stewart] |
|
Wednesday June 12 afternoon |
1. Observation of crystal growth / Seeding [T. Bergfors] 2. Experimental phasing: practical considerations [J. Basquin] 3. "Random" Microseeding [P. Pachl, P. Shaw Stewart] 4. Capillary protein crystallization using counter-diffusion techniques [J. Gavira] 5. AlphaFold and biochemical considerations for protein crystallization [Joe Ng] 6. From the biomolecule solution to its 3D structure in a microfluidic chip [C. Sauter] Optional exercises 1-2 Crystallization under oil [L. Govada] |
|
Wednesday June 12 evening |
Visit of town Ceske Budejovice and the evening in own direction |
|
Thursday June 13 morning |
8:30-9:00 Using Fluorescence to Find Your Crystals [Crissy L. Tarver] 9:00-9:45 Crystallization Screening Results Analysis and Condition Prediction [Marc L. Pusey] 9:45-10:30 Crystallographic fragment-screening: workflow, tools and procedures [Manfred Weiss] 10:30 Coffee and tea time 10:45-11:30 Preparation of protein samples for crystallization experiments [Pavlína Řezáčová] 11:30-12:15 Introduction to single particle analysis by cryo-EM [Oksana Degtjarik] 12:15-13:00 Sample preparation for single particle cryo-EM [Iuliia Iermak] |
|
Thursday June 13 afternoon |
1. Dynamic light scattering [K. Dierks] 2. Trace Fluorescent Labeling and Low Cost Fluorescent Imaging [M. Pusey, C. Tarver] 3. Crystal observation, testing, handling, mounting and cryocooling [ J. Brynda, P. Pachl] 4. Intracellular protein crystallization [L. Redecke] 5. Soaking and co-crystallization [B. Kaščáková] 6. Single particle cryo-EM [H. Luecke] Optional exercises 1-2 "Random" Microseeding [P. Pachl, P. Shaw Stewart] |
|
Thursday June 13 evening |
20:00 Discussion with speakers and tutors of the day + posters section II Theory of X-ray diffraction II. [Jeroen Mesters] Poster prize awards |
|
Friday June 14 morning |
8:30-9:15 Extremely brilliant X-ray sources and new opportunities in macromolecular crystallography [Petr Pachl] 9:15-10:00 Optimisation of crystal growth for neutron crystallography [Monika Budayová - Spano] 10:00-10:45 Structure-based drug discovery using cryoEM [Hartmut Luecke] 10:45 Coffee and tea time 11:00-11:05 3 students awarded by poster prize will give 5min lectures Student presentation 1 11:05-11:10 Student presentation 2 11:10-11:15 Student presentation 3 11:20-11:40 Integration of AFM and other optical techniques [Dušan Novotný] 11:40-12:10 Assessing the diffraction quality of crystals [Vernon Smith] |
|
Friday June 14 afternoon |
14:00-14:45 Preparation and crystallization of protein complexes: Tricks and examples from our host-virus studies [Ivana Nemčovičová] 15:30 Coffee and tea time 15:30-16:15 16:30-19:00 Final work in the lab |
|
Friday June 14 evening |
19:30 Closing ceremony followed by Farewell dinner |
|
Saturday June 15 morning |
Optional exercises: Crystal observation, testing, handling, mounting and cryocooling [P. Pachl, J. Brynda] Conventional techniques and crystallization of own proteins [J. Mesters] Intracellular protein crystallization [L. Redecke] Trace Fluorescent Labeling and Low Cost Fluorescent Imaging [M. Pusey, C. Tarver] AlphaFold and biochemical considerations for protein crystallization [Joe Ng] 12:00 Lunch take away and End of the course |
FEBS has twin commitments to high-quality publications and the promotion of molecular biosciences. As a charitable academic organization,
FEBS uses income from the journals to fund its diverse activities, including support for FEBS Advanced Courses.
Federation of European Biochemical Societies
A charitable organization advancing research in the
molecular life sciences across Europe and beyond.
© Copyright Federation of European Biochemical Societies 2023. All rights reserved.